#homologous-recombination

#homologous-recombination

DNA damage can originate from internal sources like metabolic by-products or normal cellular activities, as well as external factors such as cosmic radiation, diet, and pollution.

Recent advancements in long-read sequencing technology and assembly algorithms have enabled the generation of high-quality diploid genome assemblies, which provide a more comprehensive view of complex variants and enhance our understanding of their formation mechanisms and functional consequences.

Using CRISPR-Cas9 and adeno-associated virus (AAV)-mediated homology-directed repair, we targeted CAR integration into the endogenous human TCR alpha locus (TRAC). TRAC-CAR T cells display dynamic CAR expression that delays exhaustion and improves tumour control in xenograft and immunocompetent models. This work has been critical for the development of allogeneic CAR T cell therapy, as it disrupts the TCR after transgene insertion—a necessary step to limit graft-versus-host disease.

Colossal Biosciences, valued at $10.2bn after raising hundreds of millions of dollars in funding from investors including celebrities spanning from Tiger Woods to Paris Hilton, has provoked a stampede of acclaim as well as denunciation after announcing last year it had made the dire wolf, a species lost from the world for more than 10,000 years, de-extinct via the birth of three new pups.

Scientists in the laboratory of Rendong Yang, PhD, associate professor of Urology, have developed a new large language model that can interpret transcriptomic data in cancer cell lines more accurately than conventional approaches, as detailed in a recent study published in Nature Communications. Long-read RNA sequencing technologies have transformed transcriptomics research by detecting complex RNA splicing and gene fusion events that have often been missed by conventional short-read RNA-sequencing methods.

Biology is undergoing a transformation. After centuries of studying life as it evolves naturally, researchers are now using a combination of computation and genome engineering to intervene, generating new proteins and even whole bacteria from scratch. The use of artificial-intelligence tools to design biological components, an approach known as generative biology, is set to turbocharge this area of research. Just last year, scientists used AI-assisted design to produce artificial genes that can be expressed in mammalian cells.

Martschenko's argument is largely that genetic research and data have almost always been used thus far as a justification to further entrench extant social inequalities. But we know the solutions to many of the injustices in our world-trying to lift people out of poverty, for example-and we certainly don't need more genetic research to implement them. Trejo's point is largely that more information is generally better than less.

A virus that sickens marine mammals has been detected in Arctic waters for the first time. Scientists used drones armed with petri dishes to collect samples of blow - the air and mucus whales expel from their blowholes - from whales in northern Norway. The team identified cetacean morbillivirus in samples from humpback whales ( Megaptera novaeangliae) and one sperm whale ( Physeter macrocephalus), though the humpbacks showed no symptoms of disease.